DETERMINATION OF ENDOTHELIUM-DERIVED NITRITE NITRATE BY GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY USING (N-15)NANO2 AS INTERNAL STANDARD

被引：37

作者：

GUTZKI, FM ^{[1
]}

TSIKAS, D ^{[1
]}

ALHEID, U ^{[1
]}

FROLICH, JC ^{[1
]}

机构：

[1] HANOVER MED SCH,DEPT CLIN PHARMACOL,W-3000 HANNOVER 61,GERMANY

来源：

BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 02期

关键词：

D O I：

10.1002/bms.1200210207

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A sensitive gas chromatographic/tandem mass spectrometric method for the determination of nitrite and nitrate in endothelial cell column perfusates was developed. Nitrite/nitrate were converted in the presence of the internal standard (N-15)NaNO2 to the corresponding 1-nitro-2,4,6-trimethoxybenzene (NTMB) derivatives which were subsequently analysed by gas chromatography/tandem mass spectrometry, monitoring m/z = 46 and m/z = 47, respectively. The good reproducibility, the wide linear range of the calibration curve from 25 ng ml-1 to 2500 ng ml-1 nitrite and the low detection limit of 200 pg NTMB allowed a sensitive and accurate quantification of nitrite/nitrate in perfusates of endothelial cell columns. Bradykinin had a stimulating effect on human and bovine endothelial cells and led to a threefold increase of nitrite/nitrate in endothelial cell column perfusates compared to those of unstimulated cells.

引用

页码：97 / 102

页数：6

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