HUMAN PLACENTAL CHOLESTEROL SIDE-CHAIN CLEAVAGE - ENZYMATIC-SYNTHESIS OF (22R)-20-ALPHA,22-DIHYDROXYCHOLESTEROL

被引：22

作者：

TUCKEY, RC

CAMERON, KJ

机构：

[1] Department of Biochemistry, University of Western Australia, Nedlands

来源：

STEROIDS | 1993年 / 58卷 / 05期

关键词：

STEROIDS; CYTOCHROME-P450(SCC); PLACENTAL MITOCHONDRIA; (22R)-20-ALPHA; 22-DIHYDROXYCHOLESTEROL; (22R)-22-HYDROXYCHOLESTEROL; PREGNENOLONE;

D O I：

10.1016/0039-128X(93)90024-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(22R)-20alpha,22-Dihydroxycholesterol is the second intermediate in the conversion of cholesterol to pregnenolone by cytochrome P450scc in steroidogenic tissues. We report a rapid method for the enzymatic synthesis of (22R)-20alpha,22-dihydroxycholesterol from (22R)-22-hydroxycholesterol using mitochondria from the human placenta.

引用

页码：230 / 233

页数：4

共 14 条

[1] SIDE-CHAIN CLEAVAGE OF HYDROXYLATED STEROLS BY BOVINE ADRENAL-CORTEX MITOCHONDRIA - OBSERVATION OF 25-HYDROXYLASE ACTIVITY DURING INCUBATION AT PH 7.80
ALSEMA, GJ
DEGENHART, HJ
HOOGERBRUGGE, J
[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1980, 13 (05) : 539 - 543
[2] INTERMEDIATES IN CONVERSION OF CHOLESTEROL TO PREGNENOLONE - KINETICS AND MECHANISM
BURSTEIN, S
GUT, M
[J]. STEROIDS, 1976, 28 (01) : 115 - 131
[3] BURSTEIN S, 1975, J BIOL CHEM, V250, P9028
[4] SYNTHESIS AND STEREOCHEMISTRY OF (22R)-20ALPHA,22-DIHYDROXYCHOLESTEROL (22S)-20ALPHA,22-DIHYDROXYCHOLESTEROL
CHAUDHURI, NK
NICKOLSON, R
KIMBALL, H
GUT, M
[J]. STEROIDS, 1970, 15 (04) : 525 - +
[5] HIKINO H, 1975, CHEM PHARM BULL, V23, P1458
[6] HOCHBERG RB, 1974, J BIOL CHEM, V249, P1277
[7] HOYTE RM, 1979, J BIOL CHEM, V254, P2278
[8] THE CATALYTIC CYCLE OF CYTOCHROME-P-450SCC AND INTERMEDIATES IN THE CONVERSION OF CHOLESTEROL TO PREGNENOLONE
HUME, R
KELLY, RW
TAYLOR, PL
BOYD, GS
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (03): : 583 - 591
[9] EVIDENCE FOR 20, 22-EPOXYCHOLESTEROL AS AN INTERMEDIATE IN SIDE-CHAIN CLEAVAGE OF 22R-OH CHOLESTEROL BY ADRENAL-CORTEX MITOCHONDRIA
KRAAIPOEL, RJ
DEGENHART, HJ
VANBEEK, V
LEEUWBOON, HD
ABELN, G
VISSER, HKA
LEFERINK, JG
[J]. FEBS LETTERS, 1975, 54 (02) : 172 - 179
[10] LAMBETH JD, 1980, J BIOL CHEM, V255, P8282

← 1 2 →