A SUBPOPULATION OF STRIATAL GABAERGIC NEURONS EXPRESSES THE EPIDERMAL GROWTH-FACTOR RECEPTOR

Epidermal growth factor and transforming growth factor alpha are mitogenic polypeptides that act at the epidermal growth factor receptor, a protein tyrosine kinase.(10,16,18,24) Studies have shown that epidermal growth factor and transforming growth factor alpha support the survival and promote the differentiation of central nervous system neurons in vitro.(13,21,33) Messenger RNAs for both transforming growth factor alpha and the epidermal growth factor receptor have been identified in the adult and developing mammalian central nervous system, particularly within the neostriatum of young animals.(11,15,22,27,28,30) However, the cell types that synthesize these messenger RNAs in striatum are not well understood. The present study investigates the hypothesis that epidermal growth factor receptor and transforming growth factor alpha are synthesized by striatal GABAergic neurons using double-labeling in situ hybridization in the rat, Most neurons within the neostriatum that intensely expressed messenger RNA for the 67,000 mol. wt isoform of glutamate decarboxylase also expressed messenger RNA for the epidermal growth factor receptor. Scattered striatal cells with neuronal morphology were immunoreactive for epidermal growth factor receptor protein, indicating that epidermal growth factor receptor messenger RNA expressed by striatal neurons is translated, Striatal neurons that expressed high levels of the 67,000 mel. wt isoform of glutamate decarboxylase messenger RNA did not appear to express transforming growth factor alpha messenger RNA. The present study indicates that epidermal growth factor receptor is synthesized by subpopulation of GABAergic striatal neurons, supporting the hypothesis that transforming growth factor alpha and epidermal growth factor act directly upon neurons to produce their neurotrophic effects. These neurons may be GABAergic interneurons, which have been shown to be relatively resistant to degeneration in Huntington's disease and excitotoxic models of this disease.(6,7,9)