MUTAGENESIS OF BRUCELLA-ABORTUS - COMPARATIVE EFFICIENCY OF 3 TRANSPOSON DELIVERY SYSTEMS

被引：28

作者：

SANGARI, F ^{[1
]}

AGUERO, J ^{[1
]}

机构：

[1] UNIV CANTABRIA, FAC MED, DEPT BIOL MOLEC, CARDENAL HERRERA ORIA S-N, E-39011 SANTANDER, SPAIN

来源：

MICROBIAL PATHOGENESIS | 1991年 / 11卷 / 06期

关键词：

BRUCELLA-ABORTUS; SUICIDE PLASMIDS; TRANSPOSITION;

D O I：

10.1016/0882-4010(91)90040-H

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Three transposon-containing 'suicide' plasmids were evaluated for their efficiency in promoting insertional mutagenesis in Brucella abortus. All three plasmids are mobilizable vectors and so were conjugally transferred from mobilizing strains of the donor Escherichia coli. The transposition frequencies were expressed as the ratio of Kmr exconjugants per total recipient cells at the initiation of mating. The pUT/Km-based delivery system, which utilizes a derivative of Tn5, yielded a very high frequency of 2.7 × 10-5 of transposition in B. abortus. In comparison to previously reported accounts of transposition in B. abortus, both the over thousand-fold higher and more stable number of mutants, makes the pUT/Km system a very useful tool for in vivo transposon mutagenesis of Brucella strains. © 1991.

引用

页码：443 / 446

页数：4

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