CONSERVATION ANALYSIS OF RAT AND HUMAN SP-A GENE IDENTIFIES 5' FLANKING SEQUENCES OF RAT SP-A THAT BIND RAT LUNG NUCLEAR PROTEINS

被引:20
作者
KOURETAS, D
KARINCH, AM
RISHI, A
MELCHERS, K
FLOROS, J
机构
[1] PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT CELLULAR & MOLEC PHYSIOL,POB 850,HERSHEY,PA 17033
[2] BYK GULDEN,CONSTANCE,GERMANY
[3] HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115
关键词
D O I
10.3109/01902149309064359
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
As an initial step toward understanding regulation of tissue-specific expression of SP-A, 5' flanking sequences of the rat SP-A gene and human SP-A I gene were cloned, sequenced, and compared using dot matrix analysis. Two regions were identified, each with a considerable degree of homology between the two species. One region was proximal to the TATAA box, at position -225/-17 in rats and -226/-36 in humans, and the other at position -1115/-1026 in rats and -938/-851 in humans. Studies in rats revealed the specific binding of rat lung nuclear proteins to each of the conserved 5' flanking regions identified in rat SP-A. Binding studies using the rat proximal (rPPS) or distal (rDPS) promoter segments, or overlapping fragments of these segments, with rat nuclear extracts detected the presence of a number (1-4) of lung-specific DNA/protein complexes. When nuclear proteins from liver, a nonexpressing tissue, were used the binding profile of certain nuclear proteins differed from that of the lung. These studies, taken together, suggest that sequences within identified conserved DNA segments in the 5' flanking region of the rat SP-A gene contribute to its tissue-specific expression in rats.
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页码:485 / 503
页数:19
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共 37 条
  • [1] BALLARD P L, 1988, Pediatric Research, V23, p497A
  • [2] REGULATION OF PULMONARY SURFACTANT APOPROTEIN SP 28-36 GENE IN FETAL HUMAN-LUNG
    BALLARD, PL
    HAWGOOD, S
    LILEY, H
    WELLENSTEIN, G
    GONZALES, LW
    BENSON, B
    CORDELL, B
    WHITE, RT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) : 9527 - 9531
  • [3] THE PROTEIN ID - A NEGATIVE REGULATOR OF HELIX-LOOP-HELIX DNA-BINDING PROTEINS
    BENEZRA, R
    DAVIS, RL
    LOCKSHON, D
    TURNER, DL
    WEINTRAUB, H
    [J]. CELL, 1990, 61 (01) : 49 - 59
  • [4] BOGGARAM V, 1989, J BIOL CHEM, V264, P11421
  • [5] RABBIT LUNG SURFACTANT PROTEIN-A GENE - IDENTIFICATION OF A LUNG-SPECIFIC DNASE-I HYPERSENSITIVE SITE
    CHEN, Q
    BOGGARAM, V
    MENDELSON, CR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (06): : L662 - L671
  • [6] DEMELLO DE, 1989, AM J PATHOL, V134, P1285
  • [7] RAT PULMONARY SURFACTANT PROTEIN-A IS EXPRESSED AS 2 DIFFERENTLY SIZED MESSENGER-RNA SPECIES WHICH ARISE FROM DIFFERENTIAL POLYADENYLATION OF ONE TRANSCRIPT
    FISHER, JH
    EMRIE, PA
    SHANNON, J
    SANO, K
    HATTLER, B
    MASON, RJ
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 950 (03) : 338 - 345
  • [8] FLOROS J, 1986, J BIOL CHEM, V261, P9029
  • [9] FLOROS J, 1986, J BIOL CHEM, V261, P828
  • [10] POSTNATAL STIMULATION OF RAT SURFACTANT PROTEIN-A SYNTHESIS BY DEXAMETHASONE
    FLOROS, J
    PHELPS, DS
    HARDING, HP
    CHURCH, S
    WARE, J
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (02): : L137 - L143