PROTEIN STABILITY EFFECTS OF A COMPLETE SET OF ALANINE SUBSTITUTIONS IN ARE REPRESSOR

被引：121

作者：

MILLA, ME ^{[1
]}

BROWN, BM ^{[1
]}

SAUER, RT ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 08期

关键词：

D O I：

10.1038/nsb0894-518

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The equilibrium stabilities of a complete set of single alanine-substitution mutants of the Arc repressor of bacteriophage P22 have been determined by thermal and urea denaturation experiments. Only half the alanine substitutions cause significant changes in stability, with the most deleterious mutations affecting side chains in the hydrophobic core or in salt bridges and hydrogen bonds which are protected from solvent. The five mutations that are most destabilizing affect a cluster of core residues that seem to form a structural foundation for Arc.

引用

页码：518 / 523

页数：6

共 22 条

[1] ALBER T, 1989, ANNU REV BIOCHEM, V58, P765, DOI 10.1146/annurev.biochem.58.1.765
[2] PROTEIN STABILITY CURVES
BECKTEL, WJ
SCHELLMAN, JA
[J]. BIOPOLYMERS, 1987, 26 (11) : 1859 - 1877
[3] NUCLEAR-MAGNETIC-RESONANCE SOLUTION STRUCTURE OF THE ARC REPRESSOR USING RELAXATION MATRIX CALCULATIONS
BONVIN, AMJJ
VIS, H
BREG, JN
BURGERING, MJM
BOELENS, R
KAPTEIN, R
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (01) : 328 - 341
[4] IDENTIFYING DETERMINANTS OF FOLDING AND ACTIVITY FOR A PROTEIN OF UNKNOWN STRUCTURE
BOWIE, JU
SAUER, RT
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) : 2152 - 2156
[5] EQUILIBRIUM DISSOCIATION AND UNFOLDING OF THE ARC REPRESSOR DIMER
BOWIE, JU
SAUER, RT
[J]. BIOCHEMISTRY, 1989, 28 (18) : 7139 - 7143
[6] STRUCTURE OF ARC REPRESSOR IN SOLUTION - EVIDENCE FOR A FAMILY OF BETA-SHEET DNA-BINDING PROTEINS
BREG, JN
VANOPHEUSDEN, JHJ
BURGERING, MJM
BOELENS, R
KAPTEIN, R
[J]. NATURE, 1990, 346 (6284) : 586 - 589
[7] ARC REPRESSOR IS TETRAMERIC WHEN BOUND TO OPERATOR DNA
BROWN, BM
BOWIE, JU
SAUER, RT
[J]. BIOCHEMISTRY, 1990, 29 (51) : 11189 - 11195
[8] SCANNING MUTAGENESIS OF THE ARC REPRESSOR AS A FUNCTIONAL PROBE OF OPERATOR RECOGNITION
BROWN, BM
MILLA, ME
SMITH, TL
SAUER, RT
[J]. NATURE STRUCTURAL BIOLOGY, 1994, 1 (03): : 164 - 168
[9] DILL KA, 1991, ANNU REV BIOCHEM, V60, P795, DOI 10.1146/annurev.biochem.60.1.795
[10] PRINCIPLES OF PROTEIN STABILITY DERIVED FROM PROTEIN ENGINEERING EXPERIMENTS
FERSHT, AR
SERRANO, L
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (01) : 75 - 83

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