GROWTH-INHIBITION BY TRANSFORMING GROWTH FACTOR-BETA(TGF-BETA) TYPE-I IS RESTORED IN TGF-BETA-RESISTANT HEPATOMA-CELLS AFTER EXPRESSION OF TGF-BETA RECEPTOR TYPE-II CDNA

被引：209

作者：

INAGAKI, M

MOUSTAKAS, A

LIN, HY

LODISH, HF

CARR, BI

机构：

[1] UNIV PITTSBURGH,PITTSBURGH TRANSPLANTAT INST,PITTSBURGH,PA 15260

[2] WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142

[3] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 11期

关键词：

HUMAN HEPATOMA; TRANSFECTION; POLYCLONAL ANTISERA;

D O I：

10.1073/pnas.90.11.5359

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The growth of human hepatoma Hep 3B cells is potently inhibited by TGF-beta1 (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that was not inhibited in growth by TGF-beta1 at 5 ng/ml (200 pM) and that lacked TGF-beta receptor type II (TGF-betaRII) gene. Transfection of the cloned cDNA for human TGF-betaRII to this mutant cell line restored receptor expression as well as the inhibition in growth by TGF-beta1. In both wild-type and mutant cells stably transfected with TGF-betaRII cDNA, TGF-betaRII coimmunoprecipitated with TGF-beta receptor type I in the presence of ligand. These experiments provide direct evidence for the role of TGF-betaRII in the inhibitory effect of TGF-beta on growth and suggest that TGF-betaRII acts by means of a heteromeric surface complex with TGF-beta receptor type I.

引用

页码：5359 / 5363

页数：5

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