SYNTHESIS AND SOME PROPERTIES OF MODIFIED OLIGONUCLEOTIDES .2. OLIGONUCLEOTIDES CONTAINING 2'-DEOXY-2'-FLUORO-BETA-D-ARABINOFURANOSYL PYRIMIDINE NUCLEOSIDES

被引：29

作者：

KOIS, P ^{[1
]}

TOCIK, Z ^{[1
]}

SPASSOVA, M ^{[1
]}

REN, WY ^{[1
]}

ROSENBERG, I ^{[1
]}

SOLER, JF ^{[1
]}

WATANABE, KA ^{[1
]}

机构：

[1] CORNELL UNIV,GRAD SCH MED SCI,SLOAN KETTERING INST CANC RES,SLOAN KETTERING DIV,NEW YORK,NY 10021

来源：

NUCLEOSIDES & NUCLEOTIDES | 1993年 / 12卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/07328319308016207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to find the effects of unnatural nucleosides on the stability of duplex, several oligonucleotides containing 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-uracil(FAU), -cytosine (FAC) and -thymine (FMAU) were synthesized by two alternative approaches: phosphoramidite method on an ABI 392 synthesizer and H-phosphonate procedure on our GeneSyn I universal module synthesizer. It was shown from the melting profiles that the presence of FMAU has a large stabilizing effect on the duplex. Replacement of thymidine with FAU, or deoxycytidine with FAC resulted in the formation of less stable duplexes. Temperature-dependent CD spectroscopy demonstrated that the structures of the fluorine containing oligomers are very similar to those of unmodified oligomers.

引用

页码：1093 / 1109

页数：17

共 31 条

[1] RAS ONCOGENES - THEIR ROLE IN NEOPLASIA
BARBACID, M
[J]. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1990, 20 (03) : 225 - 235
[2] CAZENAVE C, 1991, ANTISENSE NUCLEIC AC, P47
[3] CHANG EH, 1989, ANTI-CANCER DRUG DES, V4, P221
[4] COOK PD, 1992, 10TH INT ROUND TABL
[5] ECKSTEIN F, 1991, OLIGONUCLEOTIDE SYNT
[6] ECKSTEIN F, NUCLEIC ACIDS MOL BI, V1
[7] Gait M. J, 1984, OLIGONUCLEOTIDE SYNT
[8] DUPLEX STABILITIES OF PHOSPHOROTHIOATE, METHYLPHOSPHONATE, AND RNA ANALOGS OF 2 DNA 14-MERS
KIBLERHERZOG, L
ZON, G
UZNANSKI, B
WHITTIER, G
WILSON, WD
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (11) : 2979 - 2986
[9] POLYMER-SUPPORTED RNA-SYNTHESIS AND ITS APPLICATION TO TEST THE NEAREST-NEIGHBOR MODEL FOR DUPLEX STABILITY
KIERZEK, R
CARUTHERS, MH
LONGFELLOW, CE
SWINTON, D
TURNER, DH
FREIER, SM
[J]. BIOCHEMISTRY, 1986, 25 (24) : 7840 - 7846
[10] KOSTER, 1983, TETRAHEDRON LETT, V24, P5843

← 1 2 3 4 →