A COMPARISON OF ELISA AND RPLA FOR DETECTION OF BACILLUS-CEREUS DIARRHEAL ENTEROTOXIN

被引:28
作者
DAY, TL
TATANI, SR
NOTERMANS, S
BENNETT, RW
机构
[1] UNIV MINNESOTA,DEPT FOOD SCI & NUTR,ST PAUL,MN 55108
[2] RIVM,BILTHOVEN,NETHERLANDS
[3] US FDA,WASHINGTON,DC 20204
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 77卷 / 01期
关键词
D O I
10.1111/j.1365-2672.1994.tb03037.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fourteen strains of Bacillus cereus isolated from different sources were examined for their ability to produce diarrhoeal enterotoxin by two commercial immunoassay kits (Oxoid BCET-RPLA and Tecra ELISA) and the microslide immunodiffusion assay. One strain that was positive in monkey feedings, as well as a number of other strains isolated from diarrhoeal outbreaks, gave positive results in the ELISA and negative results in the RPLA test systems. When tested in the microslide assay, these strains produced only one antigen which formed a line of identity with the reference toxin. The results of the control toxins provided with the kits substantiated that the two commercial assays did not detect the same antigen. Cultures positive with both assay kits were shown to produce diarrhoeal enterotoxin (by a line of identity) and other antigens in the microslide immunodiffusion assay.
引用
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页码:9 / 13
页数:5
相关论文
共 18 条
[1]   CHARACTERIZATION OF THE COMPONENTS OF HEMOLYSIN-BL FROM BACILLUS-CEREUS [J].
BEECHER, DJ ;
MACMILLAN, JD .
INFECTION AND IMMUNITY, 1991, 59 (05) :1778-1784
[2]  
BENNETT RW, 1993, NETH MILK DAIRY J, V47, P105
[3]  
BENNETT RW, 1992, COMPENDIUM MEHTODS M
[4]   EVALUATION OF THE OXOID BCET-RPLA KIT FOR THE DETECTION OF BACILLUS-CEREUS DIARRHEAL ENTEROTOXIN AS COMPARED TO CELL-CULTURE CYTOTONICITY [J].
BUCHANAN, RL ;
SCHULTZ, FJ .
JOURNAL OF FOOD PROTECTION, 1992, 55 (06) :440-443
[5]  
CHRISTIANSSON A, 1993, NETH MILK DAIRY J, V47, P79
[6]   TOXIN PRODUCTION BY BACILLUS-CEREUS DAIRY ISOLATES IN MILK AT LOW-TEMPERATURES [J].
CHRISTIANSSON, A ;
NAIDU, AS ;
NILSSON, I ;
WADSTROM, T ;
PETTERSSON, HE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (10) :2595-2600
[7]  
GLATZ B A, 1974, Infection and Immunity, V10, P299
[8]   EXTRACELLULAR FACTOR SYNTHESIZED BY BACILLUS-CEREUS WHICH EVOKES A DERMAL REACTION IN GUINEA-PIGS [J].
GLATZ, BA ;
GOEPFERT, JM .
INFECTION AND IMMUNITY, 1973, 8 (01) :25-29
[9]   ANALYSIS OF ENTEROTOXIN PRODUCTION BY BACILLUS-CEREUS FROM DAIRY-PRODUCTS, FOOD POISONING INCIDENTS AND NONGASTROINTESTINAL INFECTIONS [J].
GRANUM, PE ;
BRYNESTAD, S ;
KRAMER, JM .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1993, 17 (04) :269-279
[10]   TOXIN PRODUCTION BY PSYCHROTROPHIC BACILLUS SPP PRESENT IN MILK [J].
GRIFFITHS, MW .
JOURNAL OF FOOD PROTECTION, 1990, 53 (09) :790-792