FLUOROMETRIC ASSAY OF ALCOHOLS USING ALCOHOL OXIDASE

被引：12

作者：

GUILBAULT, GG

SADAR, SH

机构：

[1] Chemistry Department, Louisiana State University in New Orleans, New Orleans, Louisiana

来源：

ANALYTICAL LETTERS | 1969年 / 2卷 / 01期

关键词：

alcohol oxidase; allyl alcohol; ethanol; fluorescence; methanol; n-propanol;

D O I：

10.1080/00032716908051270

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorometric methods are described for the assay of ethanol, methanol, allyl alcohol and n-propanol using the enzyme alcohol oxidase. The enzyme catalyzes the oxidation of the alcohols to aldehydes with concomitant formation of hydrogen peroxide. The hydrogen peroxide formed is measured fluorometrically using p-hydroxy-phenylacetic acid and peroxidase. From 0.1–100 μg/ml of the alcohols and from 0.0002 to 0.050 unit/ml of alcohol oxidase can be assayed in 2 minutes with a precision of about ± 2%. © 1969, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：41 / +

页数：1

共 6 条

[1]

BOYER PD, 1963, ENZYMES ED, V7, P26

[2] NEW SUBSTRATES FOR FLUOROMETRIC DETERMINATION OF OXIDATIVE ENZYMES [J].