Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique

被引：32

作者：

Lethias, Claire ^{[1
]}

Hartmann, Daniel J. ^{[2
]}

Masmejean, Michele ^{[3
]}

Ravazzola, Mariella ^{[4
]}

Sabbagh, Issam ^{[5
]}

Ville, Gerard ^{[2
]}

Herbage, Daniel ^{[3
]}

Eloy, Rosy ^{[1
]}

机构：

[1] INSERM, Unit 37, F-69675 Bron, France

[2] Inst Pasteur, Ctr Radioanal, F-69366 Lyon 07, France

[3] Ctr Rech Appl Dermobiochim, F-69007 Lyon, France

[4] Univ Geneva, Sch Med, Inst Histol & Embryol, CH-1211 Geneva 4, Switzerland

[5] Inst Pasteur, Ctr Immunochim Prot, F-69365 Lyon 2, France

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1987年 / 35卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

elastic fibers; blood vessels; immunoelectron microscopy; protein A-gold;

D O I：

10.1177/35.1.3794307

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Identification of elastic fibers at the ultrastructural level is accomplished by a post-embedding immunohistochemical technique using the protein A-colloidal gold method. Antisera against elastins from human dermis and rat aorta have been characterized by radioimmunoassay and then applied to thin sections of rat blood vessels. Two fixative solutions and two embedding media have been tested. Both antibodies bind to elastic fibers of normal arteries and veins, indicating crossreactions among organs and species. The high sensitivity of this method is demonstrated by its application to the detection of neo-elastogenesis in the intimal thickening of aortic grafts.

引用

页码：15 / 21

页数：7

共 32 条

[1] COMBINED FLUORESCENT AND ELECTRON-MICROSCOPIC STAIN FOR ELASTIC TISSUE [J].