IMPROVED PURIFICATION OF 12-LIPOXYGENASE FROM RAT BASOPHILIC LEUKEMIA-CELLS AND CONDITIONS FOR OPTIMAL ENZYME-ACTIVITY

被引：5

作者：

VANDERDONK, EMM ^{[1
]}

DUBOIS, GR ^{[1
]}

VERHAGEN, J ^{[1
]}

VELDINK, GA ^{[1
]}

VLIEGENTHART, JFG ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT BIOORGAN CHEM,PADUALAAN 8,3584 CH UTRECHT,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1074卷 / 03期

关键词：

12-LIPOXYGENASE; 13-HYDROXYOCTADECADIENOIC ACID; (RAT BASOPHILIC LEUKEMIA CELL);

D O I：

10.1016/0304-4165(91)90098-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

12-Lipoxygenase from rat basophilic leukemia cells was purified about 300-fold by protein-HPLC in a single run. Maximal 12-lipoxygenase activity was observed at pH 7.5, while the enzyme became almost inactive at pH 6 and 9. Although Ca2+ was not essential for 12-lipoxygenase activity, the partially purified enzyme was stimulated approx. 2-fold in the presence of 0.1-5.0 mM Ca2+. Contrary to 5-lipoxygenase from RBL-1 cells, 12-lipoxygenase was not inactivated by preincubation with Ca2+ for 1-10 min, nor was it stimulated by 0.1-10 mM ATP.

引用

页码：443 / 447

页数：5

共 21 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] METABOLIC PROFILE OF LINOLEIC-ACID IN PORCINE LEUKOCYTES THROUGH THE LIPOXYGENASE PATHWAY [J].