A HIGHLY SENSITIVE DEVICE FOR VISUAL DETECTION OF ANTIGENS AND ANTIBODIES BY MEANS OF LIGHT INTERFERENCE

被引：5

作者：

KAWAGUCHI, T

SHIRO, T

IWATA, K

机构：

[1] Tokyo Research Center, Teijin Ltd., 191, 4-3-2 Asahigaoka, Hino-City, tokyo

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1991年 / 3卷 / 02期

关键词：

D O I：

10.1016/0925-4005(91)80203-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive device for the visual detection of antigens and antibodies by means of light interference has been investigated. The detection is based on the change in the light interference colour after the antigen-antibody reaction of an immobilized antibody (antigen) with an antigen (antibody) in a specimen. We investigate selective staining of the antibody part by colloidal gold treatment at an optimized pH value (colloidal gold method). We have also investigated selective staining of the antigen part on the immobilized antibody by treatment with a secondary antibody bearing colloidal gold (sandwich method). Both methods are found to enhance the sensitivity. According to the colloidal gold method, less than 1 ng/ml of anti-hCG can be visually detected by using a device with immobilized hCG. With the sandwich method, 1 ng/ml of human IgG can be visually detected by using a device with immobilized anti-human IgG.

引用

页码：113 / 121

页数：9

共 20 条

[1] ENZYME IMMUNOSENSOR .2. ELECTROCHEMICAL DETERMINATION OF IGG WITH AN ANTIBODY-BOUND MEMBRANE [J].