A MICROPLATE VERSION OF THE SOS/UMU-TEST FOR RAPID DETECTION OF GENOTOXINS AND GENOTOXIC POTENTIALS OF ENVIRONMENTAL-SAMPLES

被引：125

作者：

REIFFERSCHEID, G

HEIL, J

ODA, Y

ZAHN, RK

机构：

[1] UNIV MAINZ,ARBEITSKREIS MOLEK MECH UMWELTBEDINGTER GENTOXIZITAT,W-6500 MAINZ,GERMANY

[2] AKAD WISSENSCH & LITERATUR,W-6500 MAINZ,GERMANY

[3] OSAKA PREFECTURAL INST PUBL HLTH,OSAKA 537,JAPAN

来源：

MUTATION RESEARCH | 1991年 / 253卷 / 03期

关键词：

UMUC; GENOTOXINS; MUTAGENESIS; SALMONELLA-TYPHIMURIUM (TA1535/PSK1002); SOS RESPONSE;

D O I：

10.1016/0165-1161(91)90134-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The umu-microtest is a miniaturized automated short-term test version proposed for screening of umuC-dependent mutagenic potentials of chemicals relevant to environmental pollution, river water and industrial waste water. The test is based on the SOS/umu-test and has been modified in order to allow extensive testing of environmental samples. Genetically engineered Salmonella typhimurium (TA1535/pSK1002) are incubated on a microplate rotor in a sloping position for 2 h with the test samples, followed by addition of fresh culture medium to reach a 10-fold dilution of the incubation medium. 2 h later, the activity of the beta-galactosidase, which reflects umuC induction, is determined colorimetrically. The incubation of the bacteria in the presence of the test compounds as well as the assessment of beta-galactosidase activity takes place in 96-well microplates, thus enabling simultaneous screening of large numbers of samples. Data of the genotoxic potentials are available within 8 h. Computer-controlled automation is possible by using a laboratory workstation.

引用

页码：215 / 222

页数：8

共 23 条

[1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].