STRUCTURE, GENETIC-MAPPING, AND EXPRESSION OF THE MOUSE HGF SCATTER FACTOR GENE

被引：14

作者：

LEE, CC

KOZAK, CA

YAMADA, KM

机构：

[1] NIDR,DEV BIOL LAB,BLDG 30 RM 423,BETHESDA,MD 20892

[2] NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892

来源：

CELL ADHESION AND COMMUNICATION | 1993年 / 1卷 / 02期

关键词：

HEPATOCYTE GROWTH FACTOR; SCATTER FACTOR; GENE MAPPING; RNASE PROTECTION ASSAY;

D O I：

10.3109/15419069309095686

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytokine termed hepatocyte growth factor or scatter factor (HGF/SF) has been implicated in embryonic development and liver regeneration. Mouse HGF/SF cDNA clones were obtained by screening a mouse liver cDNA library with synthetic oligonucleotides, as well as by using the method of reverse transcription-polymerase chain reaction (RT-PCR) with mRNA isolated from adult mouse brain. Sequence analysis of mouse HGF/SF cDNA clones spanning the entire coding region revealed an overall amino acid identity of 90% and 98% with human and rat HGF, respectively. Using mouse HGF antisense RNA as a probe, we observed the expression of HGF/SF mRNA in the liver, kidney, whole brain and cerebellum of adult mice as well as in day 9 and day 10 mouse embryos. The mouse HGF/SF cDNA was utilized for mapping Hgf to the centromeric region of mouse Chromosome 5 in apparent close proximity to the reeler mutation by the analysis of two multilocus crosses. The relationship of Hgf to the reeler mutation is discussed. The availability of mouse HGF/SF cDNA clones should facilitate further analysis of HGF/SF function during development.

引用

页码：101 / 111

页数：11

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