MODIFICATION OF KANAMYCIN-ESCULIN-AZIDE AGAR TO IMPROVE SELECTIVITY IN THE ENUMERATION OF FECAL STREPTOCOCCI FROM WATER SAMPLES

被引：26

作者：

AUDICANA, A

PERALES, I

BORREGO, JJ

机构：

[1] UNIV MALAGA,FAC CIENCIAS,DEPT MICROBIOL,E-29071 MALAGA,SPAIN

[2] DELEGAC TERR SANIDAD VIZCAYA,SALUD PUBL LAB,E-48071 BILBAO,SPAIN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 12期

关键词：

D O I：

10.1128/AEM.61.12.4178-4183.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Kanamycin-esculin-azide agar was modified by increasing the concentration of sodium azide to 0.4 g liter(-1) and replacing kanamycin sulfate with 5 mg of osolinic acid liter(-1). The modification, named oxolinic acid-escuiin-azide (OAA) agar, was compared with Slanetz-Bartley and KF agars by using drinking water and seawater samples, The OAA agar showed higher specificity, selectivity, and recovery efficiencies than those obtained by using the other media, In addition, no confirmation of typical colonies was needed when OAA agar was used, which significantly shortens the time of sample processing and increases the accuracy of the method.

引用

页码：4178 / 4183

页数：6

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