CHARACTERIZATION OF AN ESTABLISHED HUMAN HEPATOMA-CELL LINE CONSTITUTIVELY EXPRESSING NONSTRUCTURAL PROTEINS OF HEPATITIS-C VIRUS BY TRANSFECTION OF VIRAL CDNA

被引：39

作者：

HARADA, T

KIM, DW

SAGAWA, K

SUZUKI, T

TAKAHASHI, K

SAITO, I

MATSUURA, Y

MIYAMURA, T

机构：

[1] NATL INST HLTH,DEPT VIROL 2,SHINJUKU KU,TOKYO 162,JAPAN

[2] KURUME UNIV,SCH MED,DEPT IMMUNOL,KURUME,FUKUOKA 830,JAPAN

[3] UNIV TOKYO,INST MED SCI,MOLEC GENET LAB,MINATO KU,TOKYO 108,JAPAN

[4] NIHON VET ANIM SCI UNIV,DEPT LAB ANIM,MUSASHINO,TOKYO 180,JAPAN

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-5-1215

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A human hepatoma cell line constitutively expressing proteins of hepatitis C virus (HCV) was established by transfection with cDNA encoding part of the virus non-structural (NS) genome region. Proteins consistent with authentic processing at NS3/NS4A, NS4A/NS4B and NS4B/NS5A were identified. Pulse-chase experiments indicated that the cleavage between NS3 and NS4A occurred first and cleavage at other sites followed. Expression of specific surface antigens in response to the presence of HCV proteins was analysed by flow cytometry. A significant increase in CD26 expression was observed in cells expressing the HCV proteins. CD26 plays an important role in cellular signal transduction. Its upregulation in response to the presence of HCV proteins may play a role in viral pathology.

引用

页码：1215 / 1221

页数：7

共 35 条

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