PEPTIDYL TRANSFER RNA .6. CHEMICAL SYNTHESIS OF TRI- AND TETRAPEPTIDYL TRANSFER RNA

被引:16
作者
LAPIDOT, Y
DEGROOT, N
RAPPOPOR.S
机构
[1] Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem
关键词
D O I
10.1016/0005-2787(69)90525-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A general method for the preparation of tripeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-monomethoxytrityldipeptide and aminoacyl-tRNA. The monomethoxytrityl group is removed from the blocked peptidyl-tRNA under very mild conditions (5% dichloroacetic acid for 5 min at 4°) leaving the tRNA intact. When N-blocked carboxyl-activated tripeptide was reacted with aminoacyl-tRNA and the product formed was treated with dichloroacetic acid, a tetrapeptidyl-tRNA was obtained. The peptidyl-tRNA's described in this communication contained only bifunctional amino acids such as glycine, alanine, valine and phenylalanine. The activated N-blocked dipeptides used were either composed of two identical amino acids as in alanylalanine or of two different amino acids as in glycylphenylalanine. © 1969.
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页码:105 / &
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