COMPARISON OF LIPASE-CATALYZED ESTERIFICATION IN SUPERCRITICAL CARBON-DIOXIDE AND IN NORMAL-HEXANE

被引：76

作者：

MARTY, A ^{[1
]}

CHULALAKSANANUKUL, W ^{[1
]}

CONDORET, JS ^{[1
]}

WILLEMOT, RM ^{[1
]}

DURAND, G ^{[1
]}

机构：

[1] INST NATL SCI APPL,DEPT GENIE BIOCHIM & ALIMENTAIRE,CNRS,UA 544,AVE RANGUEIL,F-31077 TOULOUSE,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 01期

关键词：

D O I：

10.1007/BF01028485

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oleic acid esterification by ethanol has been performed by an immobilized lipase from Mucor miehei in supercritical carbon dioxide and in n-hexane as solvents. In both media, determination of apparent kinetic constants has been achieved and influence of water content has been shown to be different due to various rates of water solubilities. Stability of the lipase has been proved to be correct and similar in both solvents. Inhibition by ethanol excess has been found but is greater in n-hexane. That can explain the higher initial velocities obtained in supercritical carbon dioxide for the highest ethanol concentrations. © 1990 Kluwer Academic Publishers.

引用

页码：11 / 16

页数：6

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