THE EFFECT OF PORE-SIZE DISTRIBUTION ON THE BINDING OF PROTEINS TO POROUS RESIN BEADS

被引：6

作者：

HASEGAWA, M ^{[1
]}

KITANO, H ^{[1
]}

机构：

[1] KYOTO UNIV,DEPT POLYMER CHEM,KYOTO 606,JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 07期

关键词：

PORE SIZE DISTRIBUTION; RESIN BEADS; POROUS; PROTEIN; RESTRICTED DIFFUSION; POLYMER RESIN; PROTEIN BINDING TO;

D O I：

10.1002/bit.260370703

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of the distribution of pore radii in the resin beads on protein binding was taken into account to analyze the elution profiles of proteins from the polymer-packed column obtained by repetitive injection method. By assuming that the distribution of pore radii in the resin beads is logarithmic Gaussian, the theoretical curves obtained agreed well with the experimental data.

引用

页码：608 / 613

页数：6

共 18 条

[1]

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[2]

CARLEYSMITH S, 1980, BIOTECHNOL BIOENG, V22, P967

[3]

Chibata I, 1978, IMMOBILIZED ENZYMES

[4]

FERY JD, 1936, J GEN PHYSIOL, V20, P95