HIGH-FREQUENCY OF MALARIA-SPECIFIC T-CELLS IN NON-EXPOSED HUMANS

被引:58
作者
ZEVERING, Y
AMANTE, F
SMILLIE, A
CURRIER, J
SMITH, G
HOUGHTEN, RA
GOOD, MF
机构
[1] QUEENSLAND INST MED RES,TROP HLTH PROGRAM,HERSTON RD,BRISBANE,QLD 4029,AUSTRALIA
[2] MICROGENESYS INC,MERIDEN,CT
[3] INST MOLEC STUDIES,SAN DIEGO,CA
关键词
D O I
10.1002/eji.1830220311
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A major goal of current candidate malaria vaccines is to stimulate the expansion of clones of malaria-specific lymphocytes. We have examined the in vitro T cell responses of a group of malaria exposed and non-exposed adult Caucasian donors to recombinant circumsporozoite (CS) proteins, one of which is undergoing clinical trials, to blood-stage parasites, and to synthetic peptides copying the CS protein and defined blood-stage proteins. In nearly all individuals tested, CD4 T cell proliferation or lymphokine production occurred in response to whole parasite or CS protein stimulation, and T cells from many individuals responded to synthetic peptides. T cell responses were major histocompatibility complex-restricted, and stimulation of T cells with malaria parasites or CS protein did not appear to expand a population of T cell receptor gamma/delta-cells. Malaria-specific responses were independent of prior malaria exposure, and in some cases exceeded the magnitude of response to tetanus toxoid. Specific T cells are present in high frequency in the peripheral blood of many donors who have never been exposed to malaria. Although malaria-specific CD4 T cells play an important role in immunity, these data question whether vaccines need to stimulate such cells, and focus attention on other aspects of malaria immunity which may be more critical to a successful vaccine.
引用
收藏
页码:689 / 696
页数:8
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