ISOLATION OF A PUTATIVE CARBOXYSOMAL CARBONIC-ANHYDRASE GENE FROM THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942

被引:74
作者
YU, JW
PRICE, GD
SONG, L
BADGER, MR
机构
[1] Plant Environmental Biology Group, Res. School of Biological Science, Australian National University, Canberra City, ACT 2601
[2] Institute of Hydrobiology, Academia Sinica
关键词
D O I
10.1104/pp.100.2.794
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO2 fixation, resulting in a high CO2-requiring phenotype. We have isolated a 3.5-kb BamHI clone (pT2) that complements the Type 11 mutants, and complementation analysis with DNA subclones indicated that the complementing region was located in the 0.75-kb XhoI-Bg/II fragment. This same region hybridized to the chloroplastic carbonic anhydrase (CA) gene from spinach on Southern blots and to a mRNA of approximate 1 kb on northern blots. Restriction mapping and sequence analysis revealed that pT2 is the same as a genomic clone (pBM3.8) that complements another high CO2-requiring (temperature sensitive) mutant, C3P-O (E. Suzuki, H. Fukuzawa, S. Miyachi [1991] Mol Gen Genet 226: 401-408). Recently, a 272-amino acid open reading frame showing 22% homology with pea and spinach chloroplast CA genes was identified in clone pBM3.8 (H. Fukuzawa, E. Suzuki, Y. Komukal, S. Miyachi [1992] Proc Natl Acad Sci USA 89: 4437-4441). CA activity was detected in Escherichia coli cells transformed with subclones of pT2 (pT2-A and pT2-Al) containing the HindIII-Bg/II fragment, and the expressed CA has properties similar to those of the CA activity associated with carboxysomes purified from Synechococcus PCC7942 (G.D. Price, J.R. Coleman, M.R. Badger [1992] Plant Physiol 100: 784-793). Therefore, it is reasonable to conclude that the HindIII-Bg/II fragment codes for the carboxysomal CA gene product. The result is discussed in the context of the role that carboxysomal CA plays in the operation of the CO2-concentrating mechanism in cyanobacteria.
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页码:794 / 800
页数:7
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