PLASMINOGEN-BINDING PROTEIN ASSOCIATED WITH THE PLASMA-MEMBRANE OF CULTURED EMBRYONIC RAT NEOCORTICAL NEURONS

被引：15

作者：

NAKAJIMA, K ^{[1
]}

NAGATA, K ^{[1
]}

HAMANOUE, M ^{[1
]}

TAKEMOTO, N ^{[1
]}

SHIMADA, A ^{[1
]}

KOHSAKA, S ^{[1
]}

机构：

[1] NATL INST NEUROSCI,DEPT NEUROCHEM,TOKYO 187,JAPAN

来源：

FEBS LETTERS | 1993年 / 333卷 / 03期

关键词：

PLASMINOGEN; PLASMA MEMBRANE; NEURON; MICROGLIA; RECEPTOR;

D O I：

10.1016/0014-5793(93)80658-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the receptor-like molecule(s) for plasminogen (PGn) on the neuronal surface, the properties of binding of PGn to the plasma membrane of cultured embryonic rat neocortical neurons were investigated. [I-125]PGn was found to specifically bind to the plasma membrane depending on the incubation temperature and time. The binding was also affected strongly by ionic strength and slightly by Ca2+. Furthermore, ligand blotting analysis revealed that [I-125]PGn binds to a major protein with an apparent molecular weight of 45 kDa among plasma membrane proteins. These results suggest that the 45-kDa protein is a PGn receptor-like molecule on the neuronal surface.

引用

页码：223 / 228

页数：6

共 32 条

[1] USE OF A NOVEL RAPID PREPARATION OF FAT-CELL PLASMA-MEMBRANES EMPLOYING PERCOLL TO INVESTIGATE THE EFFECTS OF INSULIN AND ADRENALINE ON MEMBRANE-PROTEIN PHOSPHORYLATION WITHIN INTACT FAT-CELLS [J].