IDENTIFICATION OF SEQUENCE ELEMENTS IN THE HUMAN CYTOMEGALOVIRUS DNA-POLYMERASE GENE PROMOTER REQUIRED FOR ACTIVATION BY VIRAL GENE-PRODUCTS

被引:53
作者
KERRY, JA [1 ]
PRIDDY, MA [1 ]
STENBERG, RM [1 ]
机构
[1] EASTERN VIRGINIA MED SCH, DEPT MICROBIOL & IMMUNOL, NORFOLK, VA 23501 USA
关键词
D O I
10.1128/JVI.68.7.4167-4176.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To determine the mechanisms involved in the regulation of human cytomegalovirus early gene expression, we have examined the gene that encodes the viral DNA polymerase (UL54, pol). Our previous studies demonstrated that sequences required for activation of the pol promoter by immediate-early proteins are contained within a region from -128 to +20 and that cellular proteins can bind to this activation domain. In this study, we demonstrate by competition analysis that binding of cellular proteins to pol is associated with an 18-bp region containing a single copy of a novel inverted repeat, IR1. Time course analysis indicated that viral infection increased the level of protein binding to IR1, concurrent with the activation of the pol promoter. Mutation of the IR1 element abrogated binding of cellular factors to the pol promoter and reduced by threefold the activation by immediate-early proteins. Similarly, mutation of IRI rendered the promoter poorly responsive to activation by viral infection. Mutation of additional sequence elements in the pol promoter had little effect, indicating that IR1 plays the major role in pol promoter regulation. These studies demonstrate that the interaction between cellular factors and IR1 is important for the regulation of expression of the polymerase gene by viral proteins.
引用
收藏
页码:4167 / 4176
页数:10
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