RPII15 CODES FOR THE M(R)-15,000 SUBUNIT-9 OF DROSOPHILA-MELANOGASTER RNA-POLYMERASE-II

被引：2

作者：

LIU, ZH ^{[1
]}

KONTERMANN, RE ^{[1
]}

SCHULZE, RA ^{[1
]}

PETERSEN, G ^{[1
]}

BAUTZ, EKF ^{[1
]}

机构：

[1] UNIV HEIDELBERG,DEPT MOLEC GENET,D-69120 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1993年 / 335卷 / 01期

关键词：

RNA POLYMERASE II; SMALL SUBUNIT; MALTOSE-BINDING PROTEIN; FUSION PROTEIN; DROSOPHILA MELANOGASTER;

D O I：

10.1016/0014-5793(93)80442-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RPII15 gene product of Drosophila melanogaster, which has recently been identified by sequence comparison, possesses a high similarity to subunit 9 of yeast RNA polymerase II. Using the polymerase chain reaction the coding region of RPII15 was isolated from genomic DNA of adult flies. Sequence analysis shows four amino acid substitutions in comparison to the previously reported sequence. Antisera were generated against bacterially expressed RPII15 and were used for immunoblotting experiments with RNA polymerase II of Drosophila melanogaster. This analysis identified the M(r) 15,000 subunit 9 as gene product of RPII15.

引用

页码：73 / 75

页数：3

共 9 条

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