2 MOLECULES OF CYTOCHROME-C FUNCTION AS THE ELECTRON-DONORS TO P840 IN THE REACTION-CENTER COMPLEX ISOLATED FROM A GREEN SULFUR BACTERIUM, CHLOROBIUM-TEPIDUM

被引：61

作者：

OHOKA, H ^{[1
]}

KAMEI, S ^{[1
]}

MATSUBARA, H ^{[1
]}

IWAKI, M ^{[1
]}

ITOH, S ^{[1
]}

机构：

[1] NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN

来源：

FEBS LETTERS | 1995年 / 365卷 / 01期

关键词：

CYTOCHROME C; ELECTRON TRANSFER; GREEN SULFUR BACTERIA; PHOTOSYNTHESIS; REACTION CENTER; IRON-SULFUR CENTER;

D O I：

10.1016/0014-5793(95)00433-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840(+), with a half time (t(1/2)) of 110 or 340 mu s at 24 or 12 degrees C, respectively. Optical measurements under multiflash excitations indicated that two hemes function as the immediate electron donors to P840(+). SDS-PAGE analysis of the RC complex in combination with the N-terminal amino acid sequence analyses revealed five subunit bands; a core protein (65 kDa), the light harvesting bacteriochlorophyll a protein (41 kDa), a protein with 2[4Ee-4S] clusters (31 kDa), monoheme cytochrome c (22 kDa), and a 18-kDa protein whose function is unknown, The reaction center complex, thus, contains two molecules of cytochrome c per P840.

引用

页码：30 / 34

页数：5

共 24 条

[1] PHOTOSYNTHETIC REACTION CENTER GENES IN GREEN SULFUR BACTERIA AND IN PHOTOSYSTEM-1 ARE RELATED [J].