INTRACELLULAR DYNAMICS OF RICIN FOLLOWED BY FLUORESCENCE MICROSCOPY ON LIVING CELLS REVEALS A RAPID ACCUMULATION OF THE DIMERIC TOXIN IN THE GOLGI-APPARATUS

被引:12
作者
LENDARO, E
IPPOLITI, R
BELLELLI, A
BRUNORI, M
EVANGELISTA, V
GUIDARINI, D
BENEDETTI, PA
机构
[1] UNIV ROMA LA SAPIENZA,IST PASTEUR,FDN CENCI BOLOGNETTI,DIPARTIMENTO SCI BIOCHIM,I-00185 ROME,ITALY
[2] UNIV ROMA LA SAPIENZA,CNR,CTR BIOL MOLEC,I-00185 ROME,ITALY
[3] CNR,IST BIOFIS,PISA,ITALY
关键词
ENDOCYTOSIS; FLUORESCENCE MICROSCOPY; GOLGI; TOXIN;
D O I
10.1016/0014-5793(94)00255-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The intracellular dynamics of fluorescent conjugates of the toxic lectin ricin was followed by video fluorescence microscopy on living CHO cells, demonstrating that the ricin heterodimer and its isolated B chain, after binding to the plasma membrane receptors, migrate to and accumulate in the Golgi apparatus following internalization. A ricin derivative labelled with fluorescein on the A chain and rhodamine on the B chain did not display significant splitting of the A-B heterodimer during translocation of the toxin to the Golgi; this novel finding provides support for the hypothesis that further processing of ricin takes place in this cellular compartment.
引用
收藏
页码:99 / 104
页数:6
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