DYNAMICS OF RESPIRATORY-TRACT COLONIZATION BY BRANHAMELLA-CATARRHALIS IN BRONCHIECTASIS

被引：39

作者：

KLINGMAN, KL

PYE, A

MURPHY, TF

HILL, SL

机构：

[1] SUNY BUFFALO, VET AFFAIRS MED CTR, INFECT DIS SECT, BUFFALO, NY 14260 USA

[2] SUNY BUFFALO, VET AFFAIRS MED CTR, INFECT DIS SECT, BUFFALO, NY 14260 USA

[3] SUNY BUFFALO, DEPT MICROBIOL, BUFFALO, NY 14260 USA

[4] GEN HOSP, LUNG IMMUNOBIOCHEM RES LAB, BIRMINGHAM, W MIDLANDS, ENGLAND

来源：

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 1995年 / 152卷 / 03期

关键词：

D O I：

10.1164/ajrccm.152.3.7663786

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Branhamella catarrhalis is increasingly recognized as a lower respiratory tract pathogen, particularly in chronic lung diseases. This project defines a population of patients in whom the dynamics of colonization and infection caused by this organism could be studied. A method employing pulsed field gel electrophoresis (PFGE) of genomic: DNA was developed. Twenty-eight patients with bronchiectasis followed prospectively for 26.8 mo (mean) were seen monthly or bimonthly and at the time of a purulent exacerbation. Quantitative bacterial cultures were performed on sputum obtained at each visit. Six of 28 had B. catarrhalis isolated repeatedly. Viable numbers of B. catarrhalis were similar to other bacterial pathogens. Restriction fragment length polymorphism (RFLP) analysis of chromosomal DNA using PFGE was performed on 37 of the 47 isolates recovered. Each patient was colonized by two to four strains with different RFLP patterns. Duration of colonization by the same strain was 2.3 mo (mean). Strain acquisition did not correlate with exacerbation, antibiotic therapy, or season. We conclude that (I) a subset of bronchiectatic patients is colonized with B. catarrhalis, (2) RFLP is a sensitive tool to study strain acquisition, and (3) acquisition and clearance of B. catarrhalis from the respiratory tract is a dynamic process.

引用

页码：1072 / 1078

页数：7

共 26 条

[1]

Murphy T.F., Sethi S., Bacterial infection in chronic obstructive pulmonary disease, Am. Rev. Respir. Dis., 146, pp. 1067-1083, (1992)

[2]

Srinivasan G., Raff M.J., Templeton W.C., Givens S.J., Graves R.C., Melo J.C., Branhamella catarrhalis pneumonia. Report of two cases and review of the literature, Am. Rev. Respir. Dis., 123, pp. 553-555, (1981)

[3]

Nicotra B., Rivera M., Luman J.I., Wallace R.J., Branhamella catarrhalis as a lower respiratory tract pathogen in patients with chronic lung disease, Arch. Intern. Med., 146, pp. 890-893, (1986)

[4]

West M., Berk S.L., Smith J.K., Branhamella catarrhalis pneumonia, South Med. J., 75, pp. 1021-1023, (1982)

[5]

Ninane G., Joly J., Kraytman M., Bronchopulmonary infection due to Branhamella catarrhalis: 11 cases assessed by transtracheal puncture, B.M.J., 1, pp. 276-278, (1978)

[6]

Aitken J.M., Thornley P.E., Isolation of Branhamella catarrhalis from sputum and tracheal aspirate, J. Clin. Microbiol., 18, pp. 1262-1263, (1983)

[7]

McLeod D.T., Ahmad F., Capewell S., Croughan M.J., Calder M.A., Seaton A., Increase in bronchopulmonary infection due to Branhamella catarrhalis, B.M.J., 292, pp. 1103-1105, (1986)

[8]

Choo P.W., Gantz N.M., Branhamella catarrhalis pneumonia with bacteremia, South Med. J., 82, pp. 1317-1318, (1989)

[9]

Hager H., Verghese A., Alvarez S., Berk S.L., Branhamella catarrhalis respiratory infections, Rev. Infect. Dis., 9, pp. 1140-1149, (1987)

[10]

Chapman A.J., Musher D.M., Jonsson S., Clarridge J.E., Wallace R.J., Development of bactericidal antibody during Branhamella catarrhalis infection, J. Infect. Dis., 151, pp. 878-882, (1985)

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