PHYSICOCHEMICAL STUDIES OF EQUINE INFECTIOUS ANEMIA VIRUS .I. BUOYANT DENSITY OF VIRUS

The buoyant density of the equine infectious anemia (EIA) virus was determined by cesium chloride equilibrium density gradient centrifugation. Horse leukocyte cultures were used to obtain the starting viral material and also to determine infective virus titers. The EIA virus had a very broad distribution with densities between 1.14 and 1.20 g/ml and a peak at 1.16 to 1.17 g/ml. The contaminating protein was mainly localized near a density of 1.28 g/ml which indicated that the virus was clearly separated from the protein. As a result, it was concluded that this method could be applied for further virus purification. © 1969 Springer-Verlag.