A NEW METHOD FOR PROTEIN CRYSTALLIZATION USING HIGH-PRESSURE

被引：73

作者：

VISURI, K

KAIPAINEN, E

KIVIMAKI, J

NIEMI, H

LEISOLA, M

PALOSAARI, S

机构：

[1] CULTOR LTD,CTR TECHNOL,SF-02460 KANTVIK,FINLAND

[2] LAPPEENRANTA UNIV TECHNOL,DEPT CHEM TECHNOL,SF-53851 LAPPEENRANTA,FINLAND

来源：

BIO-TECHNOLOGY | 1990年 / 8卷 / 06期

关键词：

D O I：

10.1038/nbt0690-547

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Crystallization methods for proteins have been the subject of decades of development yet protein crystallization remains the limiting step in structural studies. We present here a new method for protein crystallization—based on the use of high pressure—that enabled us to accelerate dramatically the growth of glucose isom-erase crystals. We think this method may have a more general utility. © 1990 Nature Publishing Group.

引用

页码：547 / 549

页数：3

共 13 条

[1] Change of phase under pressure. I. The phase diagram of eleven substances with especial reference to the melting curve. [J].

Bridgman, PW .

PHYSICAL REVIEW, 1914, 3 (03) :153-203

[2]

BRIDGMAN PW, 1949, PHYSICS HIGH PRESSUR

[3]

CARRELL HL, 1984, J BIOL CHEM, V259, P3230

[4]

Deffet L., 1935, B SOC CHIM BELG, V44, P41

[5] NEW DIRECTIONS IN PROTEIN CRYSTAL-GROWTH [J].

DELUCAS, LJ ;

BUGG, CE .

TRENDS IN BIOTECHNOLOGY, 1987, 5 (07) :188-193

[6] PRELIMINARY INVESTIGATIONS OF PROTEIN CRYSTAL-GROWTH USING THE SPACE-SHUTTLE [J].

DELUCAS, LJ ;

SUDDATH, FL ;

SNYDER, R ;

NAUMANN, R ;

BROOM, MB ;

PUSEY, M ;

YOST, V ;

HERREN, B ;

CARTER, D ;

NELSON, B ;

MEEHAN, EJ ;

MCPHERSON, A ;

BUGG, CE .

JOURNAL OF CRYSTAL GROWTH, 1986, 76 (03) :681-693

[7] PRESSURE AND TEMPERATURE EFFECTS ON GROWTH AND METHANE PRODUCTION OF THE EXTREME THERMOPHILE METHANOCOCCUS-JANNASCHII [J].

MILLER, JF ;

SHAH, NN ;

NELSON, CM ;

LUDLOW, JM ;

CLARK, DS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :3039-3042

[8]

Morild E, 1981, Adv Protein Chem, V34, P93, DOI 10.1016/S0065-3233(08)60519-7

[9]

OSUGI J, 1969, REV PHYS CHEM JAPAN, V38, P90

[10]

SILVENTOINEN I, 1989, 10TH P S IND CRYS, P489

← 1 2 →