TEMPERATURE-DERIVATIVE SPECTROSCOPY - A TOOL FOR PROTEIN DYNAMICS

被引：82

作者：

BERENDZEN, J ^{[1
]}

BRAUNSTEIN, D ^{[1
]}

机构：

[1] UNIV ILLINOIS, DEPT PHYS, URBANA, IL 61801 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 01期

关键词：

Enthalpy distributions; Flash photolysis; Myoglobin; Nonisothermal relaxation; Substates;

D O I：

10.1073/pnas.87.1.1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A relaxation method that measures the derivative of a population with respect to temperature is introduced and used to study the recombination of CO to sperm whale myoglobin after a photolyzing flash. Measurement of the geminate process in the infrared CO-stretch bands shows distributed activation enthalpies with different distributions for each band, transitions between two bands that correspond to photolyzed ligands, and kinetic hole burning. The data are well described by gaussian enthalpy distributions; the results match and complement those of isothermal methods. The temperature-derivative technique is further used to explore the recombination of CO from outside the heme pocket.

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页码：1 / 5

页数：5

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