EXPERIMENTAL GLOMERULONEPHRITIS INDUCED BY ANTIGEN THAT BINDS TO GLOMERULAR FIBRONECTIN

被引:15
作者
COSIO, FG
MAHAN, JD
SEDMAK, DD
机构
[1] OHIO STATE UNIV,DEPT PEDIAT,COLUMBUS,OH 43210
[2] OHIO STATE UNIV,DEPT PATHOL,COLUMBUS,OH 43210
关键词
fibronectin; Glomerulonephritis; immune complexes;
D O I
10.1016/S0272-6386(12)80514-2
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
In previous studies we demonstrated that antigens with the capacity to bind to the glycoprotein fibronectin (FN), localized in the glomerular mesangium after intravenous (IV) injection. In the present study we sought to determine if the localization of FN-binding antigens in the mesangium is capable of inducing gloimerulonephritis. A group of rats were injected IV with the FN-binding antigen phenylated gelatin (DNP-GL) followed 2 hours later by rabbit anti-DNP antibodies. This experimental protocol resulted in the development of a glomerulonephritis characterized by an initial heterologous phase and a late autologous phase. Both phases of the disease were characterized by significant histologic changes, including focal segmental mesangial matrix expansion, inflammatory cell infiltration, and an increase in endothelial and mesangial cells. By immunoperoxidase we demonstrated mesangial deposition of rabbit IgG and rat C3 during the initial heterologous phase and mesangial deposition of rat IgG and C3 during the autologous phase. Significant proteinuria developed during the heterologous phase, but not during the autologous phase of the disease. By contrast, rats injected with DNP-GL alone or anti-DNP antibodies alone did not develop significant histologic glomerular changes or proteinuria. In conclusion, antigens that bind to mesangial FN are capable of inducing glomerulonephritis. This mechanism of localization of antigens in the glomeruli may be relevant to human immune complex (IC)-mediated diseases involving antigens that have the capacity to bind to FN. © 1990, National Kidney Foundation, Inc.. All rights reserved.
引用
收藏
页码:160 / 168
页数:9
相关论文
共 20 条
[1]   ELECTRICAL CHARGE - ITS ROLE IN THE PATHOGENESIS AND PREVENTION OF EXPERIMENTAL MEMBRANOUS NEPHROPATHY IN THE RABBIT [J].
ADLER, SG ;
WANG, H ;
WARD, HJ ;
COHEN, AH ;
BORDER, WA .
JOURNAL OF CLINICAL INVESTIGATION, 1983, 71 (03) :487-499
[2]   STIMULATION OF RAT MACROPHAGE INTERLEUKIN-1 SECRETION BY PLASMA FIBRONECTIN [J].
BEEZHOLD, DH ;
LAUSE, DB .
IMMUNOLOGICAL INVESTIGATIONS, 1987, 16 (05) :437-449
[3]   CIRCULATING COMPLEXES CONTAINING IGA AND FIBRONECTIN IN PATIENTS WITH PRIMARY IGA NEPHROPATHY [J].
CEDERHOLM, B ;
WIESLANDER, J ;
BYGREN, P ;
HEINEGARD, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4865-4868
[4]   BLOOD-VESSEL FIBRONECTIN INCREASES IN CONJUNCTION WITH ENDOTHELIAL CELL-PROLIFERATION AND CAPILLARY INGROWTH DURING WOUND-HEALING [J].
CLARK, RAF ;
DELLAPELLE, P ;
MANSEAU, E ;
LANIGAN, JM ;
DVORAK, HF ;
COLVIN, RB .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1982, 79 (05) :269-276
[5]  
COSIO FG, 1984, J LAB CLIN MED, V104, P867
[6]  
COSIO FG, 1987, J IMMUNOL, V138, P2587
[7]   ROLE OF FIBRONECTIN ON THE CLEARANCE AND TISSUE UPTAKE OF ANTIGEN AND IMMUNE-COMPLEXES IN RATS [J].
COSIO, FG ;
BAKALETZ, AP .
JOURNAL OF CLINICAL INVESTIGATION, 1987, 80 (05) :1270-1279
[8]   FIBRONECTIN LOCALIZATION IN THE RAT GLOMERULUS [J].
COURTOY, PJ ;
KANWAR, YS ;
HYNES, RO ;
FARQUHAR, MG .
JOURNAL OF CELL BIOLOGY, 1980, 87 (03) :691-696
[9]  
DUNN JM, 1988, KIDNEY INT, V33, P154
[10]  
GERMUTH FG, 1978, LAB INVEST, V39, P421