ZINC IS REQUIRED FOR FOLDING AND BINDING OF A SINGLE ZINC FINGER TO DNA

被引：26

作者：

LEE, MS

GOTTESFELD, JM

WRIGHT, PE

机构：

[1] Scripps Res Inst, RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

[2] MED BIOL INST, LA JOLLA, CA 92037 USA

来源：

FEBS LETTERS | 1991年 / 279卷 / 02期

关键词：

ZINC FINGER; DNA-BINDING; 2D NMR;

D O I：

10.1016/0014-5793(91)80170-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A synthetic peptide corresponding to zinc finger 31 of the Xenopus protein Xfin adopts a folded conformation in the presence of zinc. The same peptide in the absence of zinc is not folded in a stable tertiary conformation, as determined by NMR. Binding experiments have shown that the peptide binds non-specifically to DNA only in the presence of zinc. Moreover, competitive DNA binding experiments indicate interaction with 3.9 +/- 0.4 base pairs.

引用

收藏

页码：289 / 294

页数：6

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