A RAPID AND SPECIFIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THEOPHYLLINE IN BIOLOGICAL-FLUIDS

被引：19

作者：

BLANCHARD, J ^{[1
]}

HARVEY, S ^{[1
]}

MORGAN, WJ ^{[1
]}

机构：

[1] UNIV ARIZONA,COLL PHARM,CHILDRENS RES CTR,DEPT PEDIAT & PHYSIOL,DIV RESP SCI,TUCSON,AZ 85721

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1990年 / 28卷 / 06期

关键词：

D O I：

10.1093/chromsci/28.6.303

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, specific high-performance liquid chromatographic analysis of theophylline in plasma, serum, and saliva is described. Proteins present in the biological samples are precipitated with 6% perchloric acid and the clear supernatant is chromatographed on a reversed-phase column. Only 100 μL of serum is required and concentrations as low as 0.07 μg/mL can be measured accurately. Other xanthines do not interfere in the assay. Within- and betweenday variation is ≤ 2.2%. The method shows less bias and greater precision than the TDx (Abbott Diagnostics) procedure commonly used in clinical laboratories. © 1990 Oxford University Press.

引用

页码：303 / 306

页数：4

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