POLYAMINE METABOLISM DURING SEEDLING DEVELOPMENT IN RICE

被引:15
作者
BONNEAU, L
CARRE, M
DREUMONT, C
MARTINTANGUY, J
机构
[1] INRA,AMELIORIAT PLANTES STN,F-21034 DIJON,FRANCE
[2] UNIV BOURGOGNE,PHYSIOL & BIOCHIM DEV PLANTE LAB,F-21004 DIJON,FRANCE
关键词
AMINE CONJUGATES; ARGININE DECARBOXYLASE; ORYZA SATIVA; POLYAMINE INHIBITORS; POLYAMINES; SEEDLING DEVELOPMENT;
D O I
10.1007/BF00024680
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The main free amines identified during growth and development of rice seedlings were agmatine, putrescine, spermidine, diaminopropane and tyramine. Amine composition differed according to tissue and stages of development. Conjugated amines were only found in roots. We present evidence that arginine decarboxylase (ADC) regulates putrescine during the development of rice seedlings. When ADC action was blocked by DFMA (alpha-DL-difluoromethylarginine, a specific irreversible inhibitor of ADC), polyamine titers and seedling development were diminished; when agmatine or putrescine was added, normal polyamine titers and growth were restored. The effects of DFMA were concentration dependent. DFMO (alpha-DL-difluoromethylornithine, a specific irreversible inhibitor of ornithine decarboxylase or ODC) promoted growth and development at concentrations below 2 mM. This effect was probably related to its unexplained, but consistently observed slight enhancement of rice ADC. When the increase in the concentration of spermidine was prevented by CHA (cyclohexylammonium sulfate), the number of roots increased and the increase in length of leaves and roots was strongly inhibited. The addition of exogenous spermidine at the time of treatment with CHA reversed the inhibition by CHA.
引用
收藏
页码:83 / 92
页数:10
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