IACTA OF LISTERIA-IVANOVII, ALTHOUGH DISTANTLY RELATED TO LISTERIA-MONOCYTOGENES ACTA, RESTORES ACTIN TAIL FORMATION IN AN L-MONOCYTOGENES ACTA MUTANT

A gene homologous to the actA gene of Listeria monocytogenes was cloned from Listeria ivanovii (strain CLIP257) by chromosome walking starting from the ilo gene that encodes the pore-forming toxin ivanolysin, The nucleotide sequence revealed that this gene, named iactA, encodes a protein of 1,044 amino acids (IactA) comprising a central region with seven highly conserved tandem proline-rich repeats of 47 amino acids. Although IactA and ActA share an overall similar structure, these two proteins are only distantly related. Like ActA, IactA migrates aberrantly on sodium dodecyl sulfate gels, When expressed in an L. monocytogenes actA deletion mutant strain, iactA restored actin polymerization.