THE ISOLATED NEONATAL RAT-CARDIOMYOCYTE USED IN AN INVITRO MODEL FOR ISCHEMIA .2. INDUCTION OF THE 68 KDA HEAT-SHOCK PROTEIN

被引：18

作者：

TUIJL, MJM

ENHENEGOUWEN, PMPV

VANWIJK, R

VERKLEIJ, AJ

机构：

[1] Department of Molecular Cell Biology, University of Utrecht, Utrecht

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1091卷 / 03期

关键词：

HEAT SHOCK; HSP68; ISCHEMIA; INVITRO SYSTEM;

D O I：

10.1016/0167-4889(91)90190-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a model system of cultured rat cardiac cells, the expression of the heat shock protein hsp68 was studied after simulating ischemia. We observed both an increase in hsp68 synthesis, while under normal conditions hsp68 and its mRNA could not be detected. Using an antibody against hsp70 and hsp68, immunofluorescence studies showed that during 'ischemia', when hsp68 is not yet synthesized, hsp70 migrated into the nucleus. These results demonstrate that the expression of hsp68 can be used as a marker for the occurrence of ischemia. Furthermore, these findings support the fact that this in vitro system is a suitable model for the study on myocardial infarction.

引用

页码：278 / 284

页数：7

共 29 条

[1] ABNORMAL PROTEINS SERVE AS EUKARYOTIC STRESS SIGNALS AND TRIGGER THE ACTIVATION OF HEAT-SHOCK GENES [J].