ANALYSIS OF ECDYSTEROIDS BY RADIOIMMUNOASSAY - COMPARISON OF 3 DIFFERENT ANTISERA

被引：43

作者：

REUM, L

KOOLMAN, J

机构：

[1] Physiologisch-Chemisches Institut der Philipps-Universitat

来源：

INSECT BIOCHEMISTRY | 1979年 / 9卷 / 02期

关键词：

ecdysteroid pattern; ecdysteroids; Radioimmunoassay;

D O I：

10.1016/0020-1790(79)90042-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three antisera obtained with different ecdysteroid protein conjugates as antigens were studied. Antiserum DLW has the highest antibody concentration and antiserum H-21B is most sensitive for ecdysone. Antisera H-21B and ICT-1 show a high specificity for the ecdysteroid nucleus, whereas antiserum DLW is more specific for the side chain of ecdysteroids. This can be derived from cross reaction factors of ten different ecdysteroids. No antiserum is specific enough to detect ecdysone exclusively in extracts from biological material. Ecdysterone and other ecdysone metabolites are detected likewise depending on their concentration and affinity to the antibodies. It is shown that the radioimmunoassay of biological samples can lead to an overestimation of the ecdysone concentration. © 1979.

引用

页码：135 / 142

页数：8

共 30 条

[1] AN IMPROVED HIGHLY SENSITIVE BIOASSAY OF INSECT HORMONE ECDYSONE [J].