UBIQUITINATED CONJUGATES ARE FOUND IN PREPARATIONS OF SEVERAL PLANT-VIRUSES

被引：32

作者：

HAZELWOOD, D ^{[1
]}

ZAITLIN, M ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT PLANT PATHOL,ITHACA,NY 14853

来源：

VIROLOGY | 1990年 / 177卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0042-6822(90)90490-I

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recently D. D. Dunigan, R. G. Dietzgen, J. E. Schoelz, and M. Zaitlin (Virology 165, 310-312,1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of virion preparations of several other plant viruses, using anti-human ubiquitin antiserum. Based on their polyacrylamide gel migrations, plant virus-associated ubiquitin-immunoreactive proteins were considered to be possible virus structural protein-ubiquitin conjugates of the following viruses: barley stripe mosaic, brome mosaic, cowpea mosaic (two proteins), cowpea severe mosaic (two proteins), and satellite panicum mosaic. Ubiquitinated conjugates were not detected in immunoblots of preparations of cucumber mosaic virus and Cymbidium mosaic virus. The significance of ubiquitinated viral proteins remains to be determined. © 1990.

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页码：352 / 356

页数：5

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