GENOMIC DNA CAN BE USED WITH CATIONIC METHODS FOR HIGHLY EFFICIENT TRANSFORMATION OF MAIZE PROTOPLASTS

被引：29

作者：

ANTONELLI, NM ^{[1
]}

STADLER, J ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL, AMES, IA 50011 USA

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 03期

关键词：

Black Mexican Sweet; Genomic DNA; Maize protoplasts; Transformation;

D O I：

10.1007/BF00210079

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Efficient delivery of genomic DNA fragments to maize protoplasts was obtained by new methods using the polycation Polybrene or Lipofectin cationic liposomes. Stable kanamycin-resistant secondary transformants were recovered after transfection with genomic DNA from a maize cell line that had previously been tagged with the bacterial gene neomycin phosphotransferase (nptII) in a first-round transformation. The frequency of secondary transformants with nptII-homologous DNA sequences was 3% or 6% of all randomly picked microcalli after Polybrene or Lipofectin-mediated transfection, respectively. Transformation with genomic DNA by these methods may allow easy transfer of uncloned genes encoding desirable characteristics to crop species that can be regenerated from protoplasts. © 1990 Springer-Verlag.

引用

页码：395 / 401

页数：7

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