CHARACTERIZATION OF THE SURFACE-COMPOSITION OF ALKYL BONDED PHASES UNDER REVERSED-PHASE LIQUID-CHROMATOGRAPHIC CONDITIONS USING HOMOLOGS OF ALKANOATE AND PERFLUOROALKANOATE ESTERS AS SOLUTE PROBE

A thermodynamic description is presented for the retention of homologues separated under reversed-phase conditions on chemically bonded phases. This description leads to a simple equation, which considers the influence of the surface phase composition on a solute’s retention. Addittonaily, this equation Is applied to retention data for ethyl alkanoate and methyl perfluoroalkanoate esters chromatographed with either water-methanol or water-acetontrlle mobile phases on both octyl and octadecyl bonded phases. The parameters from the equation are used to interpret the effect of the surface phase composition on retention and to compare and contrast differences In Interactions between the alkanoates and perfluoroalkanoates. © 1990, American Chemical Society. All rights reserved.