FURTHER CHARACTERIZATION OF THE NONSPECIFIC ESTERASES OF THE MOSQUITO, CULEX-TARSALIS COQUILLETT

被引：8

作者：

HOUK, EJ

CRUZ, WJ

HARDY, JL

机构：

[1] Naval Biosciences Laboratory, University of California School of Public Health, Oakland

来源：

INSECT BIOCHEMISTRY | 1979年 / 9卷 / 05期

关键词：

Culex tarsalis; electrophoresis; esterases; isolelectric focusing;

D O I：

10.1016/0020-1790(79)90060-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of various parameters on the nonspecific esterase electropherograms of the Knights Landing strain of the mosquito Culex tarsalis were determined by isolelectric focusing electrophoresis in acrylamide gels. The parameters examined were substrate preference, isolation/incubation media composition, tissue distribution, aging, deoxycholate stimulation and acetylthiocholine hydrolysis. The results corroborate the identity of two major acetylcholinesterases and document a single lipase in this mosquito. The nonspecific esterases of C. tarsalis can now be classified as: one acetylesterase, one arylesterase, one lipase, two acetylcholinesterases and thirteen carboxylesterases. © 1979.

引用

页码：429 / &

共 18 条

[1] DIFFERENCES IN DISTRIBUTION OF ESTERASE BETWEEN CELL FRACTIONS OF RAT LIVER HOMOGENATES PREPARED IN VARIOUS MEDIA - RELEVANCE TO LYSOSOMAL LOCATION OF ENZYME IN INTACT CELL [J].