BIOCHEMICAL-CHARACTERIZATION OF A PUTATIVE AXONAL GUIDANCE MOLECULE OF THE CHICK VISUAL-SYSTEM

被引：205

作者：

STAHL, B ^{[1
]}

MULLER, B ^{[1
]}

VONBOXBERG, Y ^{[1
]}

COX, EC ^{[1
]}

BONHOEFFER, F ^{[1
]}

机构：

[1] PRINCETON UNIV,DEPT BIOL,PRINCETON,NJ 08544

来源：

NEURON | 1990年 / 5卷 / 05期

关键词：

D O I：

10.1016/0896-6273(90)90227-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Temporal retinal axons growing in vitro on carpets of tectal membranes are deflected by cell membranes of posterior tectum. The activity responsible for this deflection can be abolished by antibodies raised against tectal membranes and the corresponding Fab fragments. Analysis of tectal membranes by two-dimensional gel electrophoresis and immunoblotting reveals a 33 kd glycoprotein that has a higher concentration in posterior than in anterior tectum. Its expression is developmentally regulated, and it is sensitive to phosphatidylinositol-specific phospholipase C. These are properties expected for a molecule responsible for the phenomena observed in experiments on in vitro guidance of retinal axons. © 1990.

引用

页码：735 / 743

页数：9

共 24 条

[1] FAST AND SENSITIVE DETECTION OF PROTEIN AND DNA BANDS BY TREATMENT WITH POTASSIUM-PERMANGANATE [J].