STRUCTURE AND EXPRESSION OF SUBUNIT-A FROM THE BOVINE CHROMAFFIN CELL VACUOLAR ATPASE

被引:20
作者
PAN, YX
XU, J
STRASSER, JE
HOWELL, M
DEAN, GE
机构
[1] UNIV CINCINNATI,COLL MED,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267
[2] UNIV CINCINNATI,DEPT PHYSIOL & BIOPHYS,CINCINNATI,OH 45267
关键词
H+-ATPASE; BOVINE ADRENAL MEDULLA; CDNA CLONING; SUBUNIT STRUCTURE;
D O I
10.1016/0014-5793(91)81158-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Subunit A of the vacuolar H+-ATPase class is though to be responsible for the ATP hydrolysis which drives proton-pumping. We report here the cloning and sequence determination of the first mammalian cDNA encoding a bovine vacuolar ATPase subunit A from an adrenal medulla cDNA library. Northern blots of bovine adrenal medulla RNA reveal a message of approximately 3.8 kb. The predicted peptide sequence, consisting of 618 amino acids with a calculated molecular weight of 68397 daltons, is similar to the sequences of the three known subunit A proteins. beta-Galactosidase-subunit A fusion proteins were immuno-decorated by an antiserum raised to the subunit A protein from corn coleoptile vacuoles.
引用
收藏
页码:89 / 92
页数:4
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