DESIGN OF AN ARTIFICIAL SKIN .2. CONTROL OF CHEMICAL-COMPOSITION

被引：416

作者：

YANNAS, IV ^{[1
]}

BURKE, JF ^{[1
]}

GORDON, PL ^{[1
]}

HUANG, C ^{[1
]}

RUBENSTEIN, RH ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, SHRINERS BURN INST, BOSTON, MA 02115 USA

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1980年 / 14卷 / 02期

关键词：

MEMBRANES;

D O I：

10.1002/jbm.820140203

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Detailed methodology is described for the reproducible preparation of collagen-glycosaminoglycan (GAG) membranes with known chemical composition. Purified, fully native collagen from two mammalian sources is precipitated from acid dispersion by addition of chondroitin 6-sulfate. The relative amount of GAG in the coprecipitate varies with the amount of GAG added and with the pH. Since coprecipitated GAG is generally eluted from collagen fibers by physiological fluids, control of the chemical composition of membranes is arrived at by crosslinking the collagen-GAG ionic complex with glutaraldehyde, or, alternately, by use of high-temperature vacuum dehydration. Collagen-GAG membranes appear to be useful as chemically well-characterized, solid macromolecular probes of biomaterial-tissue interactions.

引用

页码：107 / 132

页数：26

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