LOCALIZATION AND CHARACTERIZATION OF ENDOTHELIN RECEPTOR-BINDING SITES IN THE RAT-BRAIN VISUALIZED BY INVITRO AUTORADIOGRAPHY

被引:123
作者
KOHZUKI, M
CHAI, SY
PAXINOS, G
KARAVAS, A
CASLEY, DJ
JOHNSTON, CI
MENDELSOHN, FAO
机构
[1] UNIV MELBOURNE, AUSTIN HOSP, DEPT MED, HEIDELBERG, VIC 3084, AUSTRALIA
[2] UNIV NEW S WALES, SCH PSYCHOL, KENSINGTON, NSW 2033, AUSTRALIA
关键词
D O I
10.1016/0306-4522(91)90162-H
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Endothelin binding sites in rat brain were mapped by quantitative in vitro autoradiography employing [I-125]endothelin-1 as radioligand. [I-125]endothelin-1 bound with high affinity and specificity to rat cerebellar sections and was potently displaced by unlabelled endothelins (endothelin-1 > endothelin-2 = endothelin-3) and sarafotoxin 6B. The highest densities of endothelin binding sites were found in the cerebellum (especially Purkinje cell layer), choroid plexus and median eminence. High densities were found in the supraoptic and paraventricular hypothalamic nuclei, anterior hypothalamic area, ventromedial hypothalamic nucleus, mammillary nuclei and glomerular layer of olfactory bulb. Moderate densities were found in many thalamic nuclei, the pretectal region, interpeduncular nucleus, suprachiasmatic nucleus, raphe nuclei, tegmental nuclei, olfactory ventricle, red nucleus, subthalamic nucleus, central gray, reticular nuclei, vestibular nuclei, oculomotor and trochlear nuclei, hypoglossal nucleus, motor trigeminal nucleus, nucleus of the trapezoid body and lateral cerebellar nucleus. Low but detectable densities of endothelin binding sites were found in medial geniculate nucleus, fields of Ammon's horn, caudate-putamen, globus pallidus, entopeduncular nucleus, substantia nigra, anterior commissure, internal capsule, anterior pituitary, median preoptic nucleus, septohypothalamic nucleus, superior colliculus and area postrema. These patterns were completely abolished by 1-mu-M unlabelled endothelin-1, -2 and -3 and sarafotoxin S6B. Brain endothelin binding sites show high affinity for endothelin-1, -2 and -3 and sarafotoxin 6B with highest affinity for endothelin-1. Endothelin binding sites show a non-vascular pattern of distribution in the brain, suggesting that the peptide may have widespread functions as a modulator of neuronal function.
引用
收藏
页码:245 / 260
页数:16
相关论文
共 30 条
  • [1] COMPETITIVE INTERACTION BETWEEN ENDOTHELIN AND SARAFOTOXIN - BINDING AND PHOSPHOINOSITIDES HYDROLYSIS IN RAT ATRIA AND BRAIN
    AMBAR, I
    KLOOG, Y
    SCHVARTZ, I
    HAZUM, E
    SOKOLOVSKY, M
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 158 (01) : 195 - 201
  • [2] AUTORADIOGRAPHICAL LOCALIZATION OF BINDING-SITES FOR PORCINE [I-125] ENDOTHELIN-1 IN HUMANS, PIGS, AND RATS - FUNCTIONAL RELEVANCE IN HUMANS
    DAVENPORT, AP
    NUNEZ, DJ
    HALL, JA
    KAUMANN, AJ
    BROWN, MJ
    [J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1989, 13 : S166 - S170
  • [3] FIRTH JD, 1988, LANCET, V2, P1179
  • [4] EFFECT OF ENDOTHELIN ON CA-2+ INFLUX, INTRACELLULAR FREE CA-2+ LEVELS AND LIGAND-BINDING TO N-TYPE AND L-TYPE CA-2+ CHANNELS IN RAT-BRAIN
    HAMILTON, MG
    FREW, R
    LUNDY, PM
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (03) : 1332 - 1338
  • [6] CELLULAR MECHANISM OF ACTION BY A NOVEL VASOCONSTRICTOR ENDOTHELIN IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS
    HIRATA, Y
    YOSHIMI, H
    TAKATA, S
    WATANABE, TX
    KUMAGAI, S
    NAKAJIMA, K
    SAKAKIBARA, S
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (03) : 868 - 875
  • [7] [I-125] ENDOTHELIN-1 BINDING-SITES - AUTORADIOGRAPHIC STUDIES IN THE BRAIN AND PERIPHERY OF VARIOUS SPECIES INCLUDING HUMANS
    HOYER, D
    WAEBER, C
    PALACIOS, JM
    [J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1989, 13 : S162 - S165
  • [8] AUTORADIOGRAPHIC VISUALIZATION OF THE BINDING-SITES FOR [I-125] ENDOTHELIN IN RAT AND HUMAN-BRAIN
    JONES, CR
    HILEY, CR
    PELTON, JT
    MOHR, M
    [J]. NEUROSCIENCE LETTERS, 1989, 97 (03) : 276 - 279
  • [9] SARAFOTOXIN, A NOVEL VASOCONSTRICTOR PEPTIDE - PHOSPHOINOSITIDE HYDROLYSIS IN RAT-HEART AND BRAIN
    KLOOG, Y
    AMBAR, I
    SOKOLOVSKY, M
    KOCHVA, E
    WOLLBERG, Z
    BDOLAH, A
    [J]. SCIENCE, 1988, 242 (4876) : 268 - 270
  • [10] ENDOTHELIN RECEPTORS IN RAT ADRENAL-GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY
    KOHZUKI, M
    JOHNSTON, CI
    CHAI, SY
    CASLEY, DJ
    ROGERSON, F
    MENDELSOHN, FAO
    [J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1989, 16 (04) : 239 - 242