A NEW VALVE FOR ZONE-ELECTROPHORETIC SAMPLE TREATMENT COUPLED ONLINE WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：15

作者：

DEBETS, AJJ

HUPE, KP

BRINKMAN, UAT

KOK, WT

机构：

[1] FREE UNIV AMSTERDAM,DEPT ANALYT CHEM,DE BOELELAAN 1083,1081 HV AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,ANALYT CHEM LAB,1018 WV AMSTERDAM,NETHERLANDS

来源：

CHROMATOGRAPHIA | 1990年 / 29卷 / 5-6期

关键词：

Column liquid chromatography; Sample treatment; Zone electrophoresis;

D O I：

10.1007/BF02317907

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The design of a new valve arrangement for zone-electrophoretic sample treatment (ZEST) coupled online with high performance liquid chromatography is described. Characteristics of this valve, such as the internal heat development as a function of the current, have been investigated. By using quinidine and desipramine as model compounds it is shown that charged compounds can be isolated from biological samples, in about 15 min, with high selectivity. The carry-over of proteins to the analytical column has been compared with the carry-over using a pre-column sample clean-up method. The detection limits of quinidine and hydroquinidine (50 ng/ml), using zone-electrophoretic sample treatment coupled with column liquid chromatography, are in the same range as with direct injections using pre-columns. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：217 / 222

页数：6

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