ANTIBODIES RAISED AGAINST DIFFERENT OLIGOPEPTIDE SEGMENTS OF HUMAN DOPAMINE-BETA-HYDROXYLASE

被引：19

作者：

NAGATSU, I

KOBAYASHI, K

FUJII, T

KOMORI, K

SEKIGUCHI, K

TITANI, K

FUJITA, K

NAGATSU, T

机构：

[1] FUJITA HLTH UNIV, SCH MED, INST COMPREHENS MED SCI, TOYOAKE, JAPAN

[2] NAGOYA UNIV, SCH MED, DEPT BIOCHEM, NAGOYA, AICHI 466, JAPAN

来源：

NEUROSCIENCE LETTERS | 1990年 / 120卷 / 02期

关键词：

OLIGOPEPTIDE; DOPAMINE-BETA-HYDROXYLASE; NORADRENERGIC NEURON; ADRENAL GLAND; HUMAN; MAMMAL; WESTERN BLOTTING; IMMUNOHISTOCHEMISTRY;

D O I：

10.1016/0304-3940(90)90023-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We raised antibodies against 3 oligopeptide segments of human dopamine-beta-hydroxylase (hDBH) corresponding to the N-terminal (hDBH-N), the intermediate (hDBH-I, and the C-terminal (hDBH-C) amino acid sequences (residues 26-43, 452-468, and 582-598), respectively. We characterized the antibodies in terms of specificity by means of Western blotting and immunohistochemistry. Anti-hDBH-N antiserum recognized DBH in the brain (noradrenergic neurons in the pons and medulla oblongata) and adrenal medulla, not only of human but also of mouse, rat and house shrew. In contrast, anti-hDBH-C antiserum recognized only human DBH. These observations suggest that the antibody raised against the hDBH-C terminal peptide may specifically recognize only human DBH.

引用

页码：141 / 145

页数：5

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