A PROGRAMMABLE MICROPROPAGATION APPARATUS USING CYCLED LIQUID-MEDIUM

被引：32

作者：

SIMONTON, W

ROBACKER, C

KRUEGER, S

机构：

[1] DEPT HORT,GEORGIA EXPT STN,GRIFFIN,GA 30223

[2] DEPT AGR ENGN,GEORGIA EXPT STN,GRIFFIN,GA 30223

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1991年 / 27卷 / 02期

关键词：

ENDOGENOUS MICROORGANISMS; SEMIAUTOMATED MICROPROPAGATION; SPATHIPHYLLUM; TISSUE CULTURE;

D O I：

10.1007/BF00041292

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An apparatus was developed that featured programmable application of liquid medium to plant cultures for micropropagation. Computer control capabilities included liquid medium introduction and medium depth within four culture vessels, medium application and removal on an assigned schedule, schedule adjustment during a culture period and medium replacement. The medium level was controlled using an accurate custom level-sensing technique consisting of thermistors and float switches. Seven-liter polycarbonate containers were modified and used as the culture vessels. Maintaining sterility was a key constraint in the development of the plant tissue culture apparatus.

引用

页码：211 / 218

页数：8

共 21 条

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