EXPRESSION OF A LACTOCOCCUS-LACTIS PHAGE RESISTANCE MECHANISM BY STREPTOCOCCUS-THERMOPHILUS

被引：37

作者：

MOINEAU, S ^{[1
]}

WALKER, SA ^{[1
]}

HOLLER, BJ ^{[1
]}

VEDAMUTHU, ER ^{[1
]}

VANDENBERGH, PA ^{[1
]}

机构：

[1] QUEST INT,SARASOTA,FL 34243

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 07期

关键词：

D O I：

10.1128/AEM.61.7.2461-2466.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 7.8-kb lactococcal plasmid pSRQ700 encodes the LlaII restriction/modification system which recognizes and cleaves the sequence 3'-GATC-5'. When the plasmid pSRQ700 is Introduced into a phage-sensitive Lactococcus lactis strain, strong phage resistance is conferred by the LlaII system. Iri this report, we Show that pSRQ700 cannot replicate in Streptococcus thermophilus. However, if cloned into the vector pNZ123, the native LlaII system is expressed and strong phage resistance is conferred to various industrial S. thermophilus strains. Resistance against phages isolated from yogurt and mozzarella wheys was observed. To our knowledge, this is the first report of increased phage resistance in S. thermophilus.

引用

页码：2461 / 2466

页数：6

共 51 条

[1]

Ackermann H., 1987, VIRUSES PROKARYOTES, V1

[2] THE SPECIES CONCEPT AND ITS APPLICATION TO TAILED PHAGES [J].